Subgingival plaque samples were collected from the first molars by means of a sterile excavating-spoon hand-instrument and then placed immediately into an

2017-03-20

Saliva is the source of mineralization for supragingival calculus, whereas the serum transudate called gingival crevicular fluid furnishes the minerals for subgingival calculus. 71,163 The calcium concentration/content in plaque is 2 to 20 times that found in saliva. 16 Early plaque of heavy calculus formers contains more calcium, three times more phosphorus, and less potassium than that of Subgingival plaque is the most important aetiological factor of periodontal disease. Specific ecologic relation between subgingival microbial species of different virulence initiates gingival inflammation and subsequently causes periodontal destruction. subgingival plaque microbiome, and explore any associations between these parameters in a cohort of patients with PD. Patients with PD only, rather than PD and RA, were chosen to avoid the confounding factors associated with RA-related treatments. Subgingival plaque and the host response to plaque causes periodontal disease. Fermentation of sugar by bacteria in plaque (results in lactate production) causes dental caries (see module 1; Dental Plaque Be Bad for more information on biofilm/plaque formation ).

Prevalence of Candida species in subgingival plaque samples was quantified as 73.3% in Group 1, 66.6% in Group 2 and 60% in Group 3 and no statistically significant differences were observed between groups. Candida albicans was the most frequently isolated species followed by Candida krusei and Candida tropicalis. subgingival plaque. Technically, local environmental changes related to orthodontic band and brackets may influence the bacterial species in periodontal plaque. However, it seems necessary to assess variations in subgingival plaque caused by orthodontic appliances. The aim of this study was to investigate changes in subgingival plaque: ( sŭb-jinji-văl plak ) Plaque on teeth below the gingival margin in gingival crevice and periodontal pockets. Saliva is the source of mineralization for supragingival calculus, whereas the serum transudate called gingival crevicular fluid furnishes the minerals for subgingival calculus.

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2014-08-05 · Subgingival plaque samples were taken from the mesio-buccal aspect of each tooth in each subject at each monitor-ing visit. Counts of 40 subgingival spe-cies were determined in each plaque sample using the checkerboard DNA-DNA hybridization technique (Socran-sky et al. 1994). After the removal of supragingival plaque. subgingival

Plaque sample collection and DNA extraction were conducted as specified in reference 27. Libraries were prepared using the NEBNext Illumina DNA library preparation kit according to manufacturer’s specifications (New England Biolabs, Ipswich, MA). Microorganism isolates from subgingival plaque sites: Paolantonio et al. 1999: Selfcontrolled study: 24: 18–22y: 16/26/12/22 or 32/42/36/46: Lateral incisor (bracket) Molar (band) Before Subgingival plaque samples were obtained from ten individuals at least 70 years old who participated in a study to assess oral health and cognitive function.

The sulcular circumferential sweep method described for detecting subgingival plaque is done by placing the probe gently into the pocket/sulcus until it reaches the epithelial attachment, and then drawing the probe horizontally with the tip remaining at the base of the pocket/sulcus.

However, the collection of saliva is simple and contains pathogens of all intraoral surfaces. Subgingival plaque sample collection. For the diseased samples, the deepest three pockets were selected and pooled. Supragingival plaque was first removed from the sample teeth with sterilized Gracey curettes and gauze. The site was then cleaned and isolated using cotton roles and air dried gently. The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria.

Supragingival plaque and tooth- associated subgingival plaque are critical in calculus formation and root caries. 28 Aug 2020 Whilst some bacteria in plaque are harmless, but some are harmful to your oral health, including gum disease and tooth decay. The food you eat, traditional techniques lead to soft tissue damage (gums), and subgingival restorative margins (leaving of foreign materials (margins) below the gum), which 2- Sampling: After removal of supragingival plaque by means of sterile cotton balls, subgingival plaque was collected on Gracey curette inserted to the depth of 13 Oct 2016 Subgingival plaque and the host response to plaque causes A diverse collection of oral microorganisms are found in dental biofilms. A small 2 Feb 2016 Showing the patient plaque collected on a periodontal probe from an Showing subgingival plaque to a patient with the use of a periodontal will collect data from the clinical examination (clinical assessment and radiological assessment) as well as non-invasive samples of subgingival plaque and Subjects will have up to 10 sites identified as "sampling sites" that will have supragingival plaque, subgingival plaque, and gingival crevicular fluid (GCF) collected 58057 #T, Prevotella multisaccharivorax, Human subgingival plaque, chronic periodontitis, Mitsuo Sakamoto, JCM, RIKEN, Saitama, Japan, 2009-04-28 Subgingival plaque samples (SPS) and stimulated saliva samples (SSS) were collected and analyzed for the presence of JP2 and non-JP2 genotypes of Aa Methods: Subgingival plaque samples were collected from CP patients ( 30 nonsmokers and 9 smokers) and healthy subjects ( 37 non- smokers and 18 Subgingival plaque samples are also collected for microbiological analysis of the microbiota with focus on hydrogen sulfide producing bacteria.
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All six paper points from each patient were pooled in a micro centrifuge tube and stored at -200C until laboratory testing. Lab procedure DNA extraction: Plaque samples were let to thaw at 2015-04-01 Sample Collection. Buccal and subgingival plaque samples were collected 1 week after the full-mouth periodontal examination. All participants were requested to refrain from food for 8 h and oral hygiene (brushing or flossing the teeth) for 12 h before sampling. Samples from all subjects were collected and stored at −80°C for subsequent In the present study, the supragingival plaque was not removed before collection of the subgingival plaque in order to simulate the conditions existing in periodontal offices when the patient is first examined.

Så används Bacterial diversity in human subgingival plaque. J Bacteriol 2001;183:3770-83.
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58057 #T, Prevotella multisaccharivorax, Human subgingival plaque, chronic periodontitis, Mitsuo Sakamoto, JCM, RIKEN, Saitama, Japan, 2009-04-28

Calcium phosphate supersaturation, certain membrane-associated components, and the degradation of nucleation inhibitors are required for initial mineralization of plaque and bacteria. Pooled subgingival plaque samples were collected and analyzed for detection of bacteria and viruses using loop‐mediated isothermal amplification. Prevalence of subgingival A. actinomycetemcomitans , HCMV, and P. gingivalis were significantly higher among aggressive periodontitis patients than periodontally healthy controls. 2017-06-02 · The subgingival plaque was collected by inserting a sterile dental curette or sterile paper points into the bottom of the gingival crevice .

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13 Oct 2016 Subgingival plaque and the host response to plaque causes A diverse collection of oral microorganisms are found in dental biofilms. A small

The test subjects were given the study Microbial analysis of subgingival plaque samples compared to that of Haririan, Hady; Andrukhov, Oleh; Bertl, Kristina; Lettner, Stefan; Kierstein, Sonja; Moritz, Microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis2014Ingår i: Journal of Periodontology, ISSN Comprehensive antimicrobial susceptibility testing of a large collection of clinical strains Subgingival plaque microbiota in Saudi Arabians after use of miswak The system is suitable for all supra- and subgingival indications, including the In modern dental treatment, the professional, mechanical removal of plaque and offers users an optimized set of instruments for modern periodontal therapy. Trapered bristles give a better subgingival plaque removal. • The high-quality bristles ensure long brush life. • Slimline handle in 3 attractive colors.